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517,807 times visiting; Rice4611 library with rich mutations provide a rich resource and genetic insights for researchers across the rice genome, enabling precise identification of mutations linked to specific traits or genes of interest in rice.
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TILLING Platform features£º |
TILLING is a targeted mutation screening technique based on EMS induced population, and is an efficient reverse genetics approach. The rice mutant library was developed through EMS mutagenesis of the Japonica rice variety Zhonghua 11 (ZH11). In theory, a mutant of any target gene in the genome can be obtained. The currently available species for external services is rice and tobacco. 1. Highly automated The platform mainly consists of four modules: Automatic Enzyme Immunoassay Analyzer TECAN; 384 Channel PCR Instrument; Automated Liquid Handler Workstation; 96-well of Fragment Analyzer ™ of Automatic Capillary Electrophoresis System. 2. Quick service The average time spent screening each gene is approximately 15-20 days. 3. Enough mutants On average, each 1.5 kb (200 bp blind spot at both ends) target fragment in the mutagenized population will obtain approximately 15 or more mutants. 4. Low cost The average cost of the fragment is 5,000 RMB, which covers the expenses for consumables and labor.
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